Briefly describe how Methyl Green/Pyronin Staining works in cell fractionation lab(1 paragraph – 20 points). Then write the colors that you expect to see in the following samples (refer to the 9th slide for centrifugation steps):
a) Cell Homogenate.
b) Pellet 1.
c) Supernatant 2.
d) Pellet 3.
e) Supernatant 3.
Write down which part of the samples get stained with which color as well (not just green+red). Example: (Pellet 2) This pellet contains mitochondria, lysosomes, and peroxisomes. Mitochondria has its own DNA, which will be stained green, and there are mitochondrial ribosomes which may have some RNA. Those parts might get stained with a light red color. Lysosomes and peroxisomes do not have any DNA or RNA, so those parts will be transparent. If the cells are infected with a virus or bacteria, only then lysosomes might give a signal since they will be degrading viruses and bacteria in the cell.
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