Introduction: A dihybrid cross is a cross between individuals that involves two pairs of contrasting traits. To Predict the results of a dihybrid, cross all possible combinations of the four alleles from each parent must be considered You will examine a dihybrid cross involving both color and texture. Purple (P), is dominate to yellow (p), and smooth texture (S) is dominant to wrinkled (s). Both parent plants are heterozygous for both traits. Review genetics and the use of Punnett squares in a biology text before doing this experiment. MATERIALS: Assume you have ear of Com. You need a heterozygous X heterozygous 9:3:3:1, purple/yellow, starchy/sweet PROCEDURE: From above please write out: 2. The crop 1. The parental (P) cross phenotype, genotype, gametes The Fl progeny Genotype and Phenotype 4. Cross between two FI 1. Selfed litestcross The Fl gametes The expected F2 results, genotype, phenotype, genotypic ratio, phenotypic ratio. 1. First let us use a Punnett square to examine the theoretical outcome of the Heterozygous X Heterozygous dihybrid cross. USE a Punnett square. REMEMBER: a phenotype is how the offspring will look. If an individual’s genotype is heterozygous, the dominant trait will be expressed in the phenotype. There are four possible phenotypes for the offspring of this cross, and both traits are in each phenotype. Now show the ratio of the phenotypes. For this, it is simply the number of possible individuals with each phenotype. 2. Now let us examine the theoretical outcome of the dihybrid test cross Now show the ratio of the phenotypes. For this, it is simply the number of possible individuals with each phenotype.
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