Instructions

Compare and contrast conventional PCR to quantitative PCRO which we used in the labs this year. Consider such things as thermocyclers, ingredients, reagent vessels, thermocycling protocols etc. to quantitative PCR (Q-PCR), both o Ings as thermocyclers, chemical POR QPCR Question 2. Data from the seven laboratory sections were collected to learn about a SNP with two allelic forms, G or A. The probes used for this experiment detect the two Alleles of the SNP shown below. The DNA strand shown is by convention of the NCBI Human Genome sequence (+ strand). Probe SNP Allele 1 5′ Vic-…………AAATGT…………MGB-NFQ 3′ 5′ Fam–……… AAGTGT………… MGB-NFQ3′ Probe SNP Allele 2 a.) Each well of the plate contains template DNA, two primers, both probes and all other reagents needed to carry out the PCR. Explain the fluorescent dye signal that will be seen upon quantitative PCR analysis for the well containing a student’s DNA who is homozygous for the G allele? b.) What about if a student is a heterozygote? What would be the Q-PCR results in this case? Explain your answer. c.) You will receive data for the Genetics class in lab. Using the class sample of successful reactions, the following results were seen. A/A homozygotes A/G Heterozygotes G/G homozygotes Calculate the allele frequencies for each of the two alleles. Which allele is the minor allele for this SNP? SHOW YOUR WORK. Free A altle = 7 @Adres, 2x(+of At+ AG) -Total altels 2 x (total simple) = 2×12 + 142-442- 507 2X (6)

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