Instructions

Many comparisons have been drawn between development in Xenopus and Zebrafish (8 marks).

A. What is the role of the embryonic shield in zebrafish?

B. What would happen if the embryonic shield was transplanted to the ventral side of a wildtype embryo?

C. You have isolated cDNA (factor X) from zebrafish embryos that resembles Goosecoid in xenopus. Using steps from the Mantra of a developmental biologist: Design a set of experiments that would address whether factor X in fish has the same function in zebrafish as Goosecoid has in frogs. Explain your methodology and indicate the outcome expected if your hypothesis was true.

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