Instructions

Practice your understanding of dilutions with the following problems: 1. You perform a serial dilution. First dilution – pipette 1 ml of culture into 9 ml of water. Second dilution – pipette 1 ml of first dilution into another 9 ml of water. Third dilution – pipette 1ml of the second dilution into 99 ml of water. Diagram this procedure. What is the total dilution? 2. You perform a serial dilution starting with a culture containing 1,000,000 organisms/ml. First dilution – pipette 1 ml of culture into 99 ml of water. Second dilution – pipette 1 ml of first dilution into another 99 ml of water. Third dilution – pipette 1ml of the second dilution into 9 ml of water. What is the total dilution at each step? What is the number of organisms/ml at each step? 3. In the previous question, you prepare pour plates from the second dilution by pipetting either 0.1 ml or 1 ml samples. How many organisms would you expect on these plates? 4. You have a culture containing 109 organisms/ml and wish to dilute it to 10 2 (100) organisms/ ml. Design a serial dilution.

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