Instructions

A geneticist is studying the role of gene Ish in the development of the tail of zebrafish, a common model system for developmental biology. To see if Tsh is involved in tail development, he measures the expression of this gene in the tail during early versus late embryo stages. First, he conducts an RNA blot hybridization (or Northern) using the Tsh gene as the probe. His results are shown below. a. (1 pt) RNA blot hybridization: Early Late Tsh Why is actin used here? It serves as a actin b. (1 pt). Next, he conducts an immunoblot and finds the results below: Early Late Why might there be differences in band intensity (dark versus light) in the Immunoblot, but not in the RNA blot Tsh hybridization? actin Given the results in a AND b, is there evidence that Tsh is involved in tail development? Yes No (circle one) c. (1 pt) His colleague continues this investigation by conducting an in situ hybridization and IF (immune fluorescence), using the same probes that were used in part a and b above on early embryo tail tissue. She accidentally has forgotten to label her results. Which image below is most likely the in situ? A/B Which the IF? A/B Defend your answers briefly below. Early embryo tail tissue, high magnification A B (four cells with visible nuclei: fluorescence is green) d. (1 pt). What antibodies must she have used in order to conduct the immunofluorescence (IF) experiment shown in part c? Primary antibody: rabbit a Secondary antibody: a rabbit

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