Instructions

Suppose you find a novel protein in zebrafish. Based on the amino acid sequence of the protein, the precursor protein contains ER target sequence, and mature protein appears to be secreted and acts as a protease involved in tissue remodeling. You decide to determine whether it is indeed a secreted protein or not by making following fusion proteins: ideal.ACBN GFP Anchor GFP Anchor GFP Anchor 4BH GFP Anchor Section A contains the portion of this protein that appears to contain amino acid sequence for ER targeting. Section B contains the portion of this protein that appears to contain amino acid sequence for secretion. GFP stands for green fluorescence protein that emits green fluorescent light when exposed to laser (tells you where the protein is). The anchor section is designed to anchor the protein to the plasma membrane, which tells you that protein actually got out of the cell. You put the plasmid containing sequence that codes for one of the fusion proteins shown above into yeast and look for expression of GFP. 1. If you have the fusion protein that has A and B in order (very first protein on the diagram), you would expect the protein to be: Sourated and anchored the m e mbrane ideal order after going through the ER 2. If you have the fusion protein that has two As (the second protein on the diagram), you would expect the protein to be: ensin in the ER 3. If you have the fusion protein that has two Bs (third protein on the diagram), you would expect the protein to be: Seurated Ana Hached to plasma membrane, not found in the ER 4. If you have the fusion protein that has A and B in reverse order very last protein on the diagram), you would expect the protein to be: Describe the process involved in proper synthesis of any proteins, based on what you have worked on the question sets:

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