Instructions

Determine the concentration of monocytogenes enumerated from the apple slice based on the information below. You must draw your serial dilution and show your mathematical calculation to receive full credit. o The apple slice (12 g) was first mixed with 108 ml of sterile 0.1% peptone water. o After, 1 ml was added to tube containing 9 ml of sterile 0.1% peptone water. Process was repeated with two additional tubes. o 1 ml was pipetted from tubes 2 and 3 onto MOX media. o Plates were incubated at 37 for 48 hours. o The pictures below show the plates from tube 2 (left) and tube 3 (right).

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