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Cell free extracts were used to translate capped and uncapped viral mRNAs in the presence of 355 methionine. (Please note, the viruses used for capped vs uncapped tre different; therefore, do not try to compare absolute values of starting RNA.) . Copyright The Meww Companies, Inc. Person required for reproduction or display (a) Capped mRNA (6) Uncapped mRNA 150 30 Cap-binding prote Cap-binding protein 100 50 10 Cap-binding protein -Cap-binding protein PSMet incorporated opm in thousands) 30 60 30 60 Time (min) (c) Capped mRNA (0) Uncapped mRNA • Cap-binding 60 protein 150 100 40 Cap-binding protein -Cap-binding protein 50 20 Cap-binding protein 0.5 1 1 2 RNA (9) he S Hecht, and AJ. Shatin Differential stimulation of capped mRNA 285 21 (e) Capped mRNA 5 Met incorpo Uncapped mRNA • Cap binding protein 150 مسمار -Cap-binding 100 protein -Cap-binding 20 50 -Cap-binding protein 0.5 1 RNA (9) Adapted from Sonenberg, N. H. Trachael S. Hecht, and AJ. Shatkin, Dinerential stimulation of capped mRNA translation in vitro by cap-binding protein Nature 285 331, 1980 protein ООООО Which of the following are accurate statements describing the data and conclusions? Choose all that apply. Cap binding protein increases the translatability of RNA that has a cap. In un-capped mRNA, addition of the cap binding protein increases the speed of protein translation Translation of un-capped RNA is unaffected by the presence of a cap. Cap binding protein increases the translatability of RNA that does not have a cap. In capped mRNA, addition of the cap binding protein increases the amount of protein translated. In capped mRNA, addition of the cap binding protein increases the speed of protein translation.

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