Instructions

Why do you stain cheek cells and name the stain used? 6. How are check cell different from plant cells in appearance 7. Are your check cells prokaryotic or Eukaryotic? Explain how you can identify their type by looking at their feature under the microscope? 8. Which structure of the human check cell stains the darkest with methylene blue stain? 9. Would the check cells normally be attached to each other? 10. Some of the check cells may have been folded or wrinkled, what does this indicate about their thickness? als and memor or in aice bath (Chili ove in a conical and fry fragile and of onion into a the DNA them down Intion of DNA from starteris 1. Place one or two strawberry in a Ziplocha 2 Smashgrind up the strawberry using your fit and flagers for 2 minutes. (this breaks open the cells) 3. Add 10ml of the extraction buffer to the bag (50ml dishwashing detergent, 15g sodium chloride, 1 liter water) 4. Keendimush the strawberry in the bag again for 1 minute ( separate components of the cell the salt in the buffer breaks up protein chains that bind wound the nucleic acids and shields the negative charges on DNA to allow precipitation, the soap dissolves the lipid (fa) part of the strawberry cell wall and nuclear membrane) 5. Assemble your flution apparatus 6. Pour the strawberry slurry into the filtration apparatus and let it drip directly into the rest ml of the 7. slowly pour cold ethanol into the tube and observe (breaks up proteins and dissolve cell membenes and precipitates the DNA) 8 dip the loop or glass rod into the tube where the strawberry extract and ethanol layers come into contact with each other and observe Finally, clean up! Rinse of all your glass wares, and dispose used solutions and waste Pictures showing DNA extraction of DNA strands pouring alcohol into the solution precipitation of more DNA that can be spooled Post lab exercise: 1. For DNA extraction, any tissue that has cells with nuclei can be used to extract? 2. Which is the substance used to precipitate DNA? 3. The substance used to separate protein from DNA is 4. What does mashing the strawberries and treating them with detergent do to their cells? 5. Which term best describes the chromosomes in strawberries? (diploid, haploid, hexaploid, octaploid)? 6. Which substance was used to break down the cell membrane of the cells? 7. If you had extracted DNA from animal cells instead of plant cells, what cell barrier would have been different? What do plant and animal cells have that bacterial cells do not? 8. Do you think that DNA from animal cells would look the same as DNA from plant cells?

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