The disadvantages of in vitro cell culture is given below, please suggest possible practices to overcome those challenges with explanations? i. The existence of only one cell type cause the absence of interactions of cells and absence of original ECM. Only two-dimensional cell growth is possible which is attributed as not being physiological. iii. Commonly applied in static conditions without mimicking the real microenvironment. iv. In two-dimensional cell growth of in vitro experimentations, use of substrates could be limited. ii.

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