Instructions

What is the purpose of the electrophoresis of DNA in general? 2. Which electrode does DNA move toward? Why? 3. Where in the gel will we find the shortest DNA fragments? Why did we need to use PCR on our DNA first? What would we expect to see in the gel if the PCI not work at all? 4. 5. What would we expect to see in the gel if the PCR worked, but restriction digest did not? 6. What is the purpose of the pBR322/BstNI marker ladder? gel? 7. What 2 things do we need to make the DNA visible in the 8. Why do we expect each lane to have only one, two or three bands?

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