Instructions

What is the approximate mass (in Daltons) of a protein containing 500 amino acids? (assume there are no other protein modifications.)

2. Why is the peptide bond planar?

A. Bulky side chains prevent free rotation around the bond.

B. It exhibits partial double-bond character, preventing rotation.

C. Hydrogen bonding between NH and C+O groups limits movement.

D. Steric hindrance between the R group, the carbonyl oxygen and the amide nitrogen.

E. All of the above.

 3. Which of the following amino acid residues would most likely be buried in the interior of a water-soluble, globular protein?

A. Aspartate D. Lysine B. Serine E. Glutamine C. Phenylalanine

 . What is the advantage of adding SDS to gel electrophoresis?

A. SDS provides the charge necessary for polypeptide chain movement in an electric field.

B. SDS is a potent reducing agent that breaks disulfide bonds in proteins.

C. SDS preserves secondary structures but disrupts tertiary structures in proteins.

D. SDS makes proteins soluble in aqueous solutions.

E. SDS allows proteins to be separated on the basis of mass.

 6. In general, which of the following is true regarding gel-filtration chromatography and SDS PAGE for a globular protein?

A. In both, small proteins move most rapidly.

B. In both, large proteins move most rapidly.

C. In SDS PAGE, large proteins move most rapidly but in gel filtration, small proteins move most rapidly.

D. In gel filtration, large proteins move most rapidly, but in SDS PAGE, small proteins move most rapidly. E. It is impossible to predict since mobility in both methods depends more than just the size of the protein.

 7. You are faced with separating two different proteins with identical mass. Protein A is a rod-like molecule with a great deal of secondary srtucture and protein B is a globular protein that is roughly spherical. What methods could be used to separate these two proteins? (check all that apply).

A. Ion-exchange chromatography

B. Two-dimensional electrophoresis

C. SDS PAGE

D. Size exclusion chromatography

E. Affinity chromatography

 8. Which of the following is correct concerning the following equilibria? (check all that apply).

CO2 + H2O H2CO3

A. An increase in the partial pressure of CO2 will result in a decrease in pH.

B. This reaction is catalyzed by carbonic anhydrase.

C. The H2CO3 dissociates to H+ and HCO3 – .

D. The majority of CO2 is transported to the lungs in the form of HCO3 – .

E. An increase in the partial pressure of CO2 indirectly results in the release of O2 from oxyhemoglobin.

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