Instructions

Which of the following statements regarding yeast two-hybrid analysis is false? (A) The goal is to bring together the DNA-binding domain and the activation domain of the yeast Gal4 protein. (B) Gal4 protein has two domains: one that binds a specific DNA sequence domain, the other activates RNA polymerase to synthesize tRNA from an adjacent gene (reporter gene). (C) A library can be set up to fuse to the Gal4 protein DNA-binding domain. (D) The interacting protein can be quickly identified by DNA sequencing of the fusion protein’s gene. E) Some false positive results occur, due to the formation of multiprotein complexes.

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