Instructions

Some amino acids are post-translationally removed from the C-terminal end of the beta- lactamase enzyme from B. imaginarium (i.e. – after it is translated and released from the ribosome, a protease chews off a some amino acids). The wild-type enzyme, which has had the amino acids removed from the C’-terminus, is 327 amino acids in length and the C-terminal amino acids are shown below aligned with the C-terminal amino acids of a frameshift mutant, which – due to the mutation is not processed at its C-terminus. The wildtype and frameshift mutant have identical coding sequences except for the insertion or deletion of a single nucleotide in the sequences encoding the C-terminal region of the protein. Use this information to answer the questions below. codon# 321 322 323 324 325 326 327 wt: 4. Ser Thr Gly Ile Pro Ile Trp-CooH mutant: Ser Thr Gly Asn Pro His Leu Glu Met Tyr Met Arg His Glu Gly-COOH A. What was the mutational event that gave rise to the frameshift mutant? (identify which codon contains the inserted or deleted nucleotide and the position within the codon where the mutation occurred. B. Deduce the number of amino acids in the wild-type enzyme prior to processing of the C-terminus and, as far as possible, the sequence of the C-terminus of the wild-type enzyme prior to processing

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