Instructions

What is the biochemical basis of the three fluorescent stains being used in the triple staining experiment (i.e. Hoechst 33342, MitoTracker and TubulinTracker)?

Fill in the gaps with the correct answer.

Hoechst 33342 stains……………… In the …………….. by binding …….. The fluorescence observed will be ………… with an emission wavelength of ……………

MitoTracker™ dye is a …………. fluorescent dye with an emission wavelength of …………. It is specific to active………..because it is only fluorescent in an ………. environment.

TubulinTracker™ dye is a ……… fluorescent dye specific to the main constituents of the …… The emission wavelength is…….
Options are:
Red, Blue, Green, 465 nm, 522 nm, 599 nm, DNA, Cytoskeleton, Mitochondria, Endoplasmic Reticulum, Nucleus, Reducing, Oxidising, In the minor groove, To CT rich regions

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