Instructions

Explain the “process of cloning” of a fragment of DNA into a commercial vector. you can choose for example how to add a cactus DNA that codes for the flowers into an imaginary Vector. ( please provide a real life example on how to use DNA cloning)

Describe all the components (and functions of those components) that the vector needs to have to obtain a good expression and explain how you will produce and prepare your fragment of interest.

Be sure to mention:

1. how your (imaginary) DNA of interest was prepared to be cloned in a vector, mentioning all the passages from the genomic DNA of the cell it came from to the preparation for the insertion in the plasmid.

2. what would you put in your imaginary commercial vector (what needs to be there)

3 the steps of the process of cloning)

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