Instructions

Explain the swelling and deswelling kinetics of the two hydrogels with respect to change in temperature and pH. State the probable molecular level mechanism(s) which can explain this phenomenon. (10 points) b) Which characteristic feature of the hydrogels is expected to change during swelling-deswelling phenomena and identify the parameter responsible for this change? Explain your answer with respect to Peppas-Merill Equation (no calculations are required). (5 points) c) You want to examine the interaction of protein with these two hydrogels. State the conditions with respect to temperature and/or pH to explain protein interactions with these hydrogels. Qualitatively predict the change in free energy (AG) in these interactions and identify if the process in kinetically or thermodynamically controlled. (10 points) d) Isoelectric point (PI) is the pH at which a particular molecule carries no net charge. PI of vascular endothelial growth factor is 9.21. If you want to encapsulate this growth factor, which hydrogel under which condition(s) would show maximum encapsulation and minimum encapsulation efficiency? Explain your answer. (5 points) e) You made a hybrid hydrogel by crosslinking copolymer 1 and 2 together in equal proportion. State the conditions with respect to temperature and/or pH to explain protein interactions with this hybrid hydrogel Compare your analysis with analysis from part C. (5 points)

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