Uses of PCR and GMOs Directions: Discuss the following: 1. Discuss how PCR may be used for the detection of disease causing pathogens in a population. (For example, it may be used to check if a patient has a dengue virus infection. This is done by using primers that are specific for complementary DNA (CDNA) sequences that correspond to the dengue viruses. If PCR amplification occurs using cDNA from a patient’s blood sample then the patient likely has dengue viruses in his/her blood.) 2. Discuss how the cloning and expression of certain genes allows for massive production of the desired product. (For example, the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients. Prior to insulin production in bacteria, insulin was harvested from other animals such as pigs.)
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