The disadvantages of in vitro cell culture is given below, please suggest possible practices to overcome those challenges with explanations? i. The existence of only one cell type cause the absence of interactions of cells and absence of original ECM. ii. Only two-dimensional cell growth is possible which is attributed as not being physiological. iii. Commonly applied in static conditions without mimicking the real microenvironment iv. In two-dimensional cell growth of in vitro experimentations, use of substrates could be limited. 9. Please list down the advantages with the biomaterials based drug delivery systems that can overcome the drawbacks of conventional tablet dosage forms by providing controlled drug delivery. Put 4 different advantages with explanations and provide the pharmacokinetic curve also by comparing them with your own wordings.

What are the 3 factors that affect DNA migration through an agarose gel

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