What is the importance of methylation in the activity of restriction endonucleases? Ο Ο Ο Methylation of DNA alters its structure, thus changing the targeting site for certain restriction endonucleases. Cells can methylate restriction endonucleases, thus activating the enzymes. Methylation of specific DNA bases provides targeting information to some restriction endonucleases. The presence of a methylated base in DNA to be cut can block endonuclease activity.

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