Which do you think came first, phototrophy or lithotrophy? Why? What do they have in common? 2. What do you think the presence of microbes that oxidize nitrogen or sulfur compounds for energy, along with those that reduce these compounds, means for the role of microbes in ecosystems? 3. What is the evidence for the endosymbiont theory that explains the rise of mitochondria in Eukarya? Did it only happen once? 4. What does the story of the Candidate Phyla Radiation tell you about the search for living microbes in the environment? What does it imply about the potential search for extraterrestrial life..

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