Instructions

Which purified genome is infectious upon transfection into cells?

A. (-)ssRNA genome since it can serve as mRNA.

B. (+)ssRNA genome and (-)ssRNA genome. (-)ssRNA genome can be infectious upon transfection since it serves as mRNA and can be readily translated.

C. (+)ssRNA genome, but (-)ssRNA genome can also be infectious upon transfection.

D. (+)ssRNA genome since it can serve as mRNA. (-)ssRNA genome can not be infectious upon transfection and it needs (+)strand synthesis and replication.

E. (+)ssRNA genome, but it first needs (-)strand synthesis and replication.

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