Instructions

A bacteria is resistant to antifungals, there are 5 R genes that are found to confer resistance. With velvet replication it is found that 32 new mutant recessive strains.

What is a strategy exploiting the sexual cycle of bacteria to determine which of the 5 genes is mutated in the new drug resistant mutants?

Using three plasmids with the wild-type version of the aforementioned R gene the mutants were transformed, and selected for transformants on ampicillin media. After incubation each plate is covered in a lawn of yeast instead of the transformant colonies that were expected. Why?

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