Instructions

You perform the gel filtration experiment and measure the activity using your nifty colorimetric assay. The following results are obtained (the elution position of molecular weight standards is superimposed on the profile) Elution Profile 10 kDa 30 kDa 50 kDa 70 kDa 100 kDa 140 120 100 © 80 Activity (U) 60 40 20 0 0 10 20 30 70 80 90 100 40 50 60 Fraction Number A) What is the estimated molecular mass of the molecules in the sample with the highest activity?

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