Instructions

How is mitosis controlled? – MPF identification Fraction no. 1 2 3 4 5 6 7 8 9 10 A. SDS gel of proteins in purified MPF Which proteins are synthesized? 97 uses S35-methionine 67 Molecular weight (KD) gels have been exposed to X-ray film to visualize radioactively labeled proteins 43 CyclinB 3 1b Inject MPF Meiosis Oocyte arrested in G2 Egg arrested in meiosis II 30 CDK1 – Induction of mitosis % 0 0 0 0 50 85 25 0 0 Phosphorylation of histone H1 assay used as a positive control for mitosis (histone H1 is known to be phosphorylated during mitosis). B. H1 kinase activity Fraction number 6 9 12 15

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