Instructions

1) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp.

Calculate the protein concentration in the sample for a tube using 0.3 mL of the 2.0 mg/mL standard BSA.

2)The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp.

Calculate the protein concentration in the assay for a tube using 0.5 mL of the 2.0 mg/mL standard BSA.

3) The Bradford protein assay is performed by mixing up to 50 μL protein sample with 1.0 mL Bradford reagent and incubating the sample 10 min at room temp.

Because of the large volume difference between sample and reagent, protein amount (μg) is convenient to use for the x axis.

Calculate the μg amount of protein in a tube using 50 μL of the 1.0 mg/mL standard BSA.

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