Instructions

I need to determine the protein concentration in a dialysate solution but am unsure how to do the calculations, any help on how to approach this would be extremely appreciated.

Use BSA as the protein standard from a 10mg/ml stock. Make a standard curve from 0-10 ug/ml BSA. To 800ul of the BSA standards add 200ul of Bradford Reagent, mix and read OD at A590. Assay the dialysate. Mix 1,2,5 and 20ul dialysate with 800ul H20. Add 200ul bradford reagent and read OD at A590.

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