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How would signaling be affected if a mutation caused a G protein to replace GOP with GTP on its own without needing to be activated by the protein-coupled receptor? The signal would not be affected and signaling would only occur when a ligand bound to the receptor. The signaling pathway would be activated even when no ligand was present. The signaling would be blocked because G protein would be active but unable to signal due to the lack of a bound ligand. The signaling would be blocked because replacement of GDP with GTP would deactivate the protein

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