Instructions

Identify the effects of replacing the Gal4 DNA‑binding domain with the DNA‑binding domain of the lac repressor.

[] The hybrid protein will tightly bind to the lac operator.

[] The hybrid protein will not recognize the upstream activating sequence (UASG) of the GAL1 promoter.

[] The cell will not metabolize galactose.

[] Allolactose will bind to the hybrid protein in the presence of lactose.

[] Lactose metabolism genes will be constitutively expressed.

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