Instructions

Discuss the upbringing of Jason from Greek and Roman Literature; is this important to his role in this work?

CD4 and CD8 are two types of white blood cells that are monitored in patients with HIV, in order to inform treatment decisions. Specific conjugate antibody markers for CD4 and CD8 are the CD4-FITC and CD8-PE fluorochromes. Upon excitation at 488 nm, PE strongly emits light in the range 550 to 590 nm and FITC strongly emits light in the range 490 to 560 nm. Design, describe and sketch a flow cytometer, incorporating a laser, photodetectors and filters so that it can quantify levels of CD4 and CD8 in samples of whole blood in terms of a percentage of total White Blood Cell count.

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