Instructions

Which of the following region in sigma factor binds to the core promoter -10 box?

Region 1
Region 2
Region 3
Region 4
Region 2 or 4 WRONG The transcription of rrn gene in E. coli can be regulated by the following mechanisms EXCEPT the presence of UP elements the binding of activators Fis to Fis sites in the upstream region of the core promoter the abundance of initiating NTP the availability of glucose sugar the presence of alarmone WRONG

Which of the following is NOT a component of the bacterial RNA polymerase core enzyme?

subunit alpha
subunit beta WRONG
subunit omega
subunit sigma
subunit beta prime

In the experiment to study the effect of temperature change on RNA polymerase binding to DNA, we found that as the temperature is _______, the binding of RNA polymerase to DNA decreases. Because _______ temperatures promote DNA melting and encourage RNA polymerase binding.

decreased, lower
decreased, higher
increased, lower WRONG
increased, higher
None of the above

To reconstitute an active rifampicin-resistant RNA polymerase, we can use the combinations of the following

rifampicin-sensitive alpha, beta, sigma and omega subunits
rifampicin-sensitive alpha and beta subunits, and rifampicin-resistant sigma and omega subunits
rifampicin-sensitive beta and sigma subunits, and rifampicin-resistant alpha and omega subunits
rifampicin-sensitive alpha and sigma subunits, and rifampicin-resistant beta and omega subunits
rifampicin-sensitive alpha, beta and sigma subunits, and rifampicin-resistant omega subunits

Which sigma factors is essential for general transcription in exponentially growing E. coli cells?

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