Instructions

Which evidence indicate a sequence of genomic DNA contains a gene?(from a-d)Use a method to sequencing an entire genome, which is true for sequencing DNA fragment.(from e-h)

a. a long sequence with a start codon in frame

b. A sequence with a start codon followed by a TATA box  

c. A palindromic sequence that is recognized by a restriction enzyme

d. a long sequence without a stop codon in frame.

e.The DNA need from more than 1 species

f. overlap fragments,  read 1 = 1-500, read 2 = 450-950

g.The DNA need be from cDNA

h. no overlap for fragment, read 1 = 1-500, read 2 = 600 -1100

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