Instructions

The Scenario: For three years a team of researchers have been watching three different Humpback whale pods off the coast of Hawaii. They are especially interested in the genetic diversity of these whales. As the number of humpback whales are decreasing, determining the genetic diversity of these populations is increasingly more important. In particular, this team is interested in identifying the parents of one of the new baby whales, Luna. Through their observations of nursing behavior they have matched Luna with her mother, however, none of their observations have helped them link Luna to her father. Today you are going to help them figure this out! Humpback Whale Pod Information: Pod Gender DNA Sample # 1 1 1 2 Female (mother) Female (Luna) Candidate Father #1 Candidate Father #2 1 2 3 4 Some questions to get you thinking about today’s lab: Q6. What is DNA and what does it do? (4 marks) Q7. What are some characteristics or properties of DNA? (4 marks) 28. What tricks can we use to see DNA? (4 marks) LAB PROCEDURE: In our hypothetical / remote lab we were provided with: Materials 6 = DNA from Mother (1), Luna (2), and the two male whales (#3 and 4) (DNA loading dye already added) Melted agarose (SYBR Safe DNA stain already added) Tris-acetate/EDTA solution (TAE) micropipette/tips electrophoresis apparatus and power supply In our hypothetical / remote lab in teams of 2 you would have been asked to: Procedure: 1. Get your electrophoresis apparatus. Make sure the combs are in place (there should be 2 combs) and that there are stoppers at both ends of the gel space. 2. Pour hot agarose into the gel space until it reaches the top of the gel box. Let the agarose harden, which should take about 10 minutes. Don’t touch/move your gel until it’s hard. 3. When the agarose gel is hard, take out the stoppers and pour solution over your gel so that it is completely covered plus a little more. 4. Gently remove the combs and stoppers. 5. Load your DNA SAMPLES into the wells near the BLACK ELECTRODE. Be sure to keep track of which samples you loaded in which lanes. 6. Run that gel!! Plug the electrodes into your gel box (red to red, black to black), being careful not to bump your gel too much. Plug the power source into an outlet and set at 125 V. (You would have) Draw a picture of your gel and label which samples are where before you add DNA to the gel. 29. What is the purpose of 9a. The loading dye? (3 marks) 9b. The DNA ladder? (3 marks) 7

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