Below is a DNA sequence of part of a hypothetical wild-type bacterial genome. The nucleotides are numbered 1 to 100. Transcription begins with and includes the T/A (top strand/botom strand) base pair at position 7 (indicated by the arrow), and proceeds from left to right along the DNA. Translation of the transcript yields the peptide of interest 10 20 30 40 50 5″ TA 5 51 60 70 80 90 100 (a) What are the first 12 nucleotides of the resulting messenger RNA (mRNA)? Indicate the 5′ and 3 ends of the mRNA (2 marks) Second Letter uuu Phe ucu UAA Stop UGA Stop A CAU His cou CUA letter AUU AUG Met ACC Arg GoU GAU Asp GGU Glu GGA Page 12 of 14

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