1. a) What four main parameters need to be optimised when optimising a cAMP assay for a new cell line?
b) In a cAMP assay, what is the purpose of forskolin?
c) In a cAMP assay, what is the purpose of IBMX?
d) Cells normally grow and are kept alive in culture media. In a cAMP assay why are cells kept in stimulation buffer rather than in culture media?
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