Instructions

A GENE221 student carried out the Ames test with histidine-requiring strains of Salmonella typhimurium (strains TA7001-TA7006) and obtained the results in the table below. Why might the plate of TA7004 with no mutagen have so many colonies compared to the other “No mutagen” plates? Number of bacterial colonies per plate TA7001 TA7002 TA7003 TA 7004 TA7003 TA7006 LATGC LTA->AD (TAG) (GOAD (CG->AD (CG>GC) No 10 $ 47 12 18 mutage Mutagen 120 TO a mutation event early in the growth of the TA7004 culture has resulted in a jackpot culture of “bacteria that can grow without histidine b. all three of the above could be true the mutation required for reversion to histidine-independence in strain TA7004 occurs at a high frequency in S. typhimurium Od the culture of TA7004 has become contaminated with bacteria that can grow without histidine 1 points Save Answer QUESTION 6 The Ames test strains can detect the following mutations: AT > GC, AT-> TA AT > CG, CG -> TA, CG-> AT, CG-> GC and frameshift mutations. A chemical sodium bananate is known to cause mutations in mice but does not affect any of the strains used in the Ames test. What is the most likely reason that it is not detected as a mutagen in the Ames test? The compound is not mutagenic for bacteria but is converted into a mutagenic compound in mice b. It causes mutations (e.g GC->TA) that are not detected in the Ames test c. Sodium bananate is not taken up by the bacterial cells d. It is toxic to the bacteria and so revertants cannot grow

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