Instructions

What is the activity and general source of restriction endonucleases? 2. Why could the treatment (digestion) of DNA from two different species with the same restriction endonucleases yield different patterns of fragments? 3. DNA fragments are seperated by agarose gel electrophoresis based on what properties of the DNA? What is used to separate the DNA fragments? 4. What does PCR stand for? What is the purpose of the technique? How is the procedure made specific for a given piece of DNA (1.e. why was only the 165 gene copied and not other pieces of the bacterial genome)? 5. How could the process of PCR be used as a molecular diagnostic tool?

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