Instructions

What is the first step in the PCR reaction cycle? denaturation b primer extension c. annealing d. cooling Q. 7. Anion exchange has 2. positively charged resin that binds to positively charged proteins (cations) * positively charged resin that binds to negatively charged proteins (anions) negatively charged resin that binds to positively charged proteins (cations) d. negatively charged resin that binds to negatively charged proteins (anions) Q. 8. Proteins are treafod with in order to denature them by interfering with hydrophobic / hydrophilic interactions. This substance also covers the entire protein with a 1. Sodium dodecyl sulfate (SDS), positive charge b. beta-mercaptoethanol (B-ME), positive charge sodium dodecyl sulfate (SDS), negative charge d. beta-mercaptoethanol (B-ME), negative charge Q. 9. In sequential order, what are the tree steps of PCR reaction cycle? anneal primers, extend DNA, denature DNA b. denature DNA, extend DNA, anneal primers denature DNA, anneal primers, extend DNA d. extend DNA, anncal primers, denature DNA Q. 10. Which of the following is NOT true about Taq DNA polymerase? 1. it adds nucleotide triphosphates to form a new DNA strand b. it requires a salt environment (buffer) to work tean act as a case to cut single-stranded DNA di fonction est mely high temperature SECTION A-MULTIPLE CHOICE 20 Marks Instruction: For each question, choose the best response. Circle the correct answer. Q.1. The Bradford assay is used to determine: the amount of protein in solution b. the type of protein in solution c. the specific amino acids in solution d. none of the above

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