Instructions

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? 1. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA. a. I, II, IV, III, V d. III, IV, V, I, II b. II, III, V, IV, 1 e. IV, V, I, II, III C. III, II, IV, V, I Figure 20.1 10. Which enzyme was used to produce the molecule in Figure 20.1? a. ligase d. RNA polymerase b. transcriptase e. DNA polymerase c. a restriction enzyme TTT A BI Figure 20.2 11. The segment of DNA shown in Figure 20.2 has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments? BAC

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