What objective lens is best when you are first starting out on a slide? Why? 2. What does ‘Par focal’ mean? Why is it useful? 3. The equation for the Numerical Aperture is NA=nsino. What does ‘n’ stand for? What does ‘o’ stand for? If we want to get a better resolution, should we increase or decrease these values? Feel free to refer back to the lecture slides. 4. Using your textbook or other sources, please describe the following types of microscopy. In your description, please briefly explain how they work and why you would use them to look at a sample. Electron microscopy Confocal microscopy Darkfield microscopy Fluorescent microscopy

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