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What type of analysis would you use to determine the size of the protein neurotoxin in Clostridium botulinum? O Microarray analysis ORFLP analysis O SDS-page O clone library analysis Which technique would be best if a researcher wanted to quantify a DNA based virus in a patient sample? Real-time – reverse transcriptase – PCR PCR and gel electrophoresis real-time PCR O northern blot Which technique would be best if a researcher wanted to quantify a RNA based virus in a patient sample? O PCR and gel electrophoresis O Real-time – reverse transcriptase – PCR real-time PCR O northern blot Which of the following is not a step or reagent involved in detecting RNA using real-time RT-PCR? O gel electrophoresis Otaq polymerase reverse transcriptase denaturation A scientist is trying to determine if several patients (1-3) are suffering from the MRSA. She extracts and purifies DNA from each patients’ isolated bacterium and performs a RFLP analysis using her lab strain of MRSA as a reference. Which of the patients, if any, are suffering from MRSA? MRSA 1 2 3 3 -I HILI IL TITULUI I MITILLITI TILIL Credit: modified “RFLP Analysises” in figure 12.15 in OpenStax Microbiology’s adapted from the NCBI is licensed under CC BY 4.0 e. patient 2 patient 3 patient 1 none

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