Instructions

Describe differential centrifugation. performed today? 1. What was the general differential centrifugation scheme you 2. Why are the fractions you isolated not considered pure but instead defined as “enriched”? A scientist recently identified a nuclear protein involved in nuclear lamina assembly. Which fraction of your differential centrifugation scheme would be the most enriched fraction for your protein of interest? Why? 3. 4. Why do you think it is important to keep the cell fractions on ice? 5. How did you detect the enrichment of mitochondria in the isolated fractions?

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