Instructions

Which of the following could you use to create a transgenic organism?

Transposable element
CRISPR-Cas9
RFLP
Two of the above
All three techniques would work

You work on the mustard plant, Arabidopsis thaliana, and wish to generate a knockout strain. Which of the following can you use to get your mutant construct into your plants that wouldn’t work if you studied fruit flies?

Transformation
Gene gun
Site-directed mutagenesis
Bacteriorphage

Bicoid ______ is tethered to the _____ end of the microtubules during oogenesis. The microtubule-associated factors recognize the ________ of the molecule.

mRNA, 3’, 5’ UTR
mRNA, negative, 3’ UTR
protein, negative, 5’ UTR
protein, positive, 3’ UTR

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