Why does chromatin need to be “remodeled” for transcription to occur? Why was it logical to observe that DNA- protein interactions can be loosened by the addidtion of acetyl groups to histones?

2) Explain the logic behind the experiment by Tonegawa and colleagues that was responsible for the discovery of enhancers. Which treatments in experiment served as controls, and what did they control for.

3) Histones proteins have been extremely highly conserved during evolution. The histones found in fruit flies and humans, for example are nearly identical in amino acid sequence. offer an explanation for this observation.

4)Explain how restriction endonuclease and DNA ligase are used to insert foreign genes into plasmids and create recombinant DNA. Illustrate some of the key events involved– including an explanation of why sticky ends are important.

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